This article discusses the validation suggestions for flow cytometry when determining precision, Limit of Blank (LOB), Limit of Detection (LOD), and Limit of Quantitation (LOQ) among other topics. This is a great resource in determining the needs to qualify a flow cytometry assay as well, and can potentially be applied to other assays if desired. This article discusses the statistical considerations as well as technical considerations when looking to validate a flow cytometry assay.
https://doi.org/10.1002/cpz1.868